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KMID : 0380020070220060438
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Korean Journal of Biotechnology and Bioengineering
2007 Volume.22 No. 6 p.438 ~ p.442
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Development of a Biosensor Using Electrochemically-Active Bacteria [EAB] for Measurements of BOD [Biochemical Oxygen Demand]
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Yoon Seok-Min
Hong Seok-Won
Choi Yong-Soo
Kim Hyung-Joo
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Abstract
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A biosensor using electrochemically-active bacteria (EAB) enriched in three-electrode electrochemical cell, was developed for the determination of biochemical oxygen demand (BOD) in wastewater. In the electrochemical cell, the positively poised working electrode with applying a potential of 0.7 V was used as an electron acceptor for the EAB. The experimental results using artificial and raw wastewater showed that the current pattern generated by the biosensor and its Coulombic yield were proportional to the concentration of organic matter in wastewater. The correlation coefficients of BOD vs Coulombic yield and vs Coulombic yield were 0.99 and 0.98, respectively. These results indicate that the biosensor enriched with the EAB capable of transferring electrons directly toward the electrode can be utilized as a water-quality monitoring system due to a quick and accurate response.
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KEYWORD
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BOD biosensor, three-electrode system, electrochemically-active- bacteria (EAB), electrochemical enrichment
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